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Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day <t>56</t> <t>post-BLM</t> (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) <t>FLIP</t> (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.
Anti Flip Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day <t>56</t> <t>post-BLM</t> (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) <t>FLIP</t> (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.
Anti Flip, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day <t>56</t> <t>post-BLM</t> (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) <t>FLIP</t> (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.
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Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day <t>56</t> <t>post-BLM</t> (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) <t>FLIP</t> (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.
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Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day <t>56</t> <t>post-BLM</t> (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) <t>FLIP</t> (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.
Flip Af821, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day 56 post-BLM (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) FLIP (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.

Journal: International Journal of Molecular Sciences

Article Title: Forefront: MiR-34a-Knockout Mice with Wild Type Hematopoietic Cells, Retain Persistent Fibrosis Following Lung Injury

doi: 10.3390/ijms21062228

Figure Lengend Snippet: Persistent fibrosis in Bleomycin-treated miR-34 KO as opposed to recovery in WT mice. ( A ) H&E staining (Upper panels), with graphical presentation of SMI-H&E (Lower panel), at day 56 post-BLM (vs. saline, inserts), in WT and miR-34 KO murine lung tissue sections. ( B ) Sircoll-collagen assay at indicated days, and ( C ) Masson-Trichrome (blue), with αSMA (brown). ( D ) FLIP (brown) IHC at day 56 post-BLM in WT, and miR-34 KO mice lung tissue sections, with ( E ) Western blot graphical presentation of FLIP levels in WT and miR-34 KO in isolated lung fibroblasts on day-56. n = 3–4 for each time point. Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p ≤ 0.01–0.05.

Article Snippet: FLIP was determined in myofibroblasts isolated from lungs of humans with IPF and from mice at different time points after OA-BLM by Western blot, as we described previously [ ] using anti-FLIP antibody (Cat. No. AF821, R&D Systems, Minneapolis, MN, USA).

Techniques: Staining, Saline, Collagen Assay, Western Blot, Isolation